POLR2C, Recombinant, Human, aa1-275, His-Tag (DNA-Directed RNA Polymerase II Subunit RPB3, HRPB33, HsRPB3, RPB3, RPB31)

Size

£512.00

SKU:: 218459
Molecular Weight:: 33.8
Purity:: ≥90% (SDS-PAGE)
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 1mM DTT, 0.1M sodium chloride, 10% glycerol.
Extra Details:: POLR2C is the third largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a cysteine rich region and exists as a heterodimer with another polymerase subunit, POLR2J. These two subunits form a core subassembly unit of the polymerase. A pseudogene has been identified on chromosome 21. Source: Recombinant protein corresponding to aa1-275 from human POLR2C, fused to His-tag at N-terminus, expressed in E.coli. Molecular Weight: ~33.8kD (298aa), confirmed by MALDI-TOF Applications: Suitable for use in SDS-PAGE. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.