PELI2, Recombinant, Human, aa1-420, His-Tag (E3 Ubiquitin-Protein Ligase Pellino Homolog 2)

Size

£512.00

SKU:: 218441
Molecular Weight:: 48.8
Purity:: ~80% (SDS-PAGE)
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 0.15M sodium chloride, 1mM DTT, 20% glycerol.
Extra Details:: PELI2 is an E3 ubiquitin ligase catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins. This protein is involved in the TLR and IL-1 signaling pathways via interaction with the complex containing IRAK kinases and TRAF6. It mediates IL1B-induced IRAK1 'Lys-63'-linked polyubiquitination and possibly 'Lys-48'-linked ubiquitination. PELI2 may be important for LPS- and IL1B-induced MAP3K7-dependent, but not MAP3K3-dependent, NF-kappa-B activation. It can activate the MAP (mitogen activated protein) kinase pathway leading to activation of ELK1. Source: Recombinant protein corresponding to aa1-420 from human PELI2, fused to His-tag at N-terminus, expressed in E.coli. Molecular Weight: ~48.8kD (443aa), confirmed by MALDI-TOF Applications: Suitable for use in SDS-PAGE. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice