NMT1, Recombinant, Human, aa1-496, His-Tag (Glycylpeptide N-Tetradecanoyltransferase 1, NMT, Myristoyl-CoA:Protein N-Myristoyltransferase 1)

Size

£448.00

SKU:: 218399
Molecular Weight:: 59.2
Purity:: ≥85% (SDS-PAGE)
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a liquid in PBS, pH 7.4. No preservative added.
Extra Details:: Myristate, a rare 14-carbon saturated fatty acid, is cotranslationally attached by an amide linkage to the N-terminal glycine residue of cellular and viral proteins with diverse functions. N-myristoyltransferase catalyzes the transfer of myristate from CoA to proteins. N-myristoylation appears to be irreversible and is required for full expression of the biologic activities of several N-myristoylated proteins, including the alpha subunit of the signal-transducing guanine nucleotide-binding protein (G protein) GO. Recombinant protein corresponding to aa1-496 from human NMT1, fused to His-tag at N-terminus, expressed in E.coli. Accession: NP_066565.1 Molecular Weight: ~59.2kD (519aa) Applications: Suitable for use in SDS-PAGE. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.