NFU1, Recombinant, Human, aa10-254, His-Tag (NFu1 Iron-Sulfur Cluster Scaffold Homolog, Mitochondrial, CGI-33, HIRIP, HIRIP5, MMDS1, Nfu, NifU, NIFUC)

Size

£512.00

SKU:: 218396
Molecular Weight:: 29.9
Purity:: ≥90% (SDS-PAGE)
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a liquid in PBS, pH 7.4, 1mM DTT, 20% glycerol.
Extra Details:: NFu1 is a protein that is localized to mitochondria and plays a critical role in iron-sulfur cluster biogenesis. This protein assembles and transfers 4Fe-4S clusters to target apoproteins including succinate dehydrogenase and lipoic acid synthase. Mutations in this gene are a cause of multiple mitochondrial dysfunctions syndrome-1, and pseudogenes of this gene are located on the short arms of chromosomes 1 and 3. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. Source: Recombinant protein corresponding to aa10-254 from human NFu1, fused to His-tag at N-terminus, expressed in E.coli. Molecular Weight: ~29.9kD (268aa) Applications: Suitable for use in SDS-PAGE. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.