NELFE, Recombinant, Human, aa1-380, His-Tag (Negative Elongation Factor E, Negative Elongation Factor Complex Member E, D6S45, NELF-E, RD, RDBP, RDP)

Size

£512.00

SKU:: 218394
Molecular Weight:: 45.6
Purity:: ~90% (SDS-PAGE)
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 0.2M sodium chloride, 5mM DTT, 50% glycerol.
Extra Details:: NELFE is a part of complex termed negative elongation factor (NELF) which represses RNA polymerase II transcript elongation. This protein bears similarity to nuclear RNA-binding proteins; however, it has not been demonstrated that this protein binds RNA. It contains a tract of alternating basic and acidic residues, largely arginine (R) and aspartic acid (D). Source: Recombinant protein corresponding to aa1-380 from human NELFE, fused to His-tag at N-terminus, expressed in E.coli. Molecular Weight: ~45.6kD (403aa), confirmed by MALDI-TOF Applications: Suitable for use in SDS-PAGE. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.