NDUFS2, Recombinant, Human, aa77-463, His-Tag (NADH Dehydrogenase [Ubiquinone] Iron-Sulfur Protein 2, Mitochondrial Isoform 1 Precursor)

Size

£512.00

SKU:: 218392
Molecular Weight:: 46.5
Purity:: ~80% (SDS-PAGE)
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 0.4M urea, 10% glycerol.
Extra Details:: NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial isoform 1 precursor, also known as NDuFS2, is a 463aa protein that is suggested to be required for catalytic activity. Defects in NDuFS2 are the cause of complex I mitochondrial respiratory chain deficiency, which is characterized by a variety of symptoms including liver failure, cardiomyopathy and neurodegeneration. Located in the mitochondrial inner membrane, mitochondrial complex I is the first and largest enzyme in the electron transport chain of oxidative phosphorylation. Source: Recombinant corresponding to aa77-463 from human NDuFS2 protein, fused to His-tag at N-terminus, expressed in E.coli. Molecular Weight: ~46.5kD (410aa) Applications: Suitable for use in SDS-PAGE. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.