NCK1 (NCK Adaptor Protein 1)

Size

£438.00

SKU:: 218384
Purity:: Purified by Protein A affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to aa1-377 from human NCK1 expressed in E. coli.
Clone:: 15B47
Format:: Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 10% glycerol.
Extra Details:: NCK1 (NCK adaptor protein 1) is a 377aa protein. NCK1 belongs to the adaptor family of proteins. It is an adaptor protein involved in transducing signals from receptor tyrosine kinases to downstream signal recipients such as RAS. NCK1 is located in the cytoplasm, but shuttles between the cytoplasm and the nucleus. Overexpression of Nck1 in NIH/3T3 cells decreases DNA synthesis stimulated by EGF. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Hybridoma: F0 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.