MTHFD2, Recombinant, Human, aa30-350, His-Tag (Bifunctional Methylenetetrahydrofolate Dehydrogenase/Cyclohydrolase, Mitochondrial)

Size

£512.00

SKU:: 218369
Molecular Weight:: 37.2
Purity:: ≥95% (SDS-PAGE)
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a liquid in PBS, pH 7.4, 1mM DTT, 20% glycerol.
Extra Details:: MTHFD2 is also known as Bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial. MTHFD2 protein possesses three distinct enzymatic activities, methylenetetrahydrofolate dehydrogenase, methenyltetrahydrofolate cyclohydrolase and formate-tetrahydrofolate ligase. Each of these activities catalyzes one of three sequential reactions in the interconversion of 1-carbon derivatives of tetrahydrofolate, which are substrates for methionine, thymidylate, and de novo purine syntheses. Source: Recombinant protein corresponding to aa30-350 from human MTHFD2, fused to His-tag at N-terminus, expressed in E.coli. Molecular Weight: ~37.2kD (344aa), confirmed by MALDI-TOF Applications: Suitable for use in SDS-PAGE. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.