MGAT2, Recombinant, Human, aa30-447, His-Tag (Mannosyl (alpha-1,6-)-Glycoprotein beta-1,2-N-Acetylglucosaminyltransferase, CDGS2, GLCNACTII, GNT-II, GNT2)

Size

£512.00

SKU:: 218338
Molecular Weight:: 50
Purity:: ≥85% (SDS-PAGE)
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 0.4M urea, 10% glycerol.
Extra Details:: MGAT2 is a golgi enzyme catalyzing an essential step in the conversion of oligomannose to complex N-glycans. The enzyme has the typical glycosyltransferase domains: a short N-terminal cytoplasmic domain, a hydrophobic non-cleavable signal-anchor domain, and a C-terminal catalytic domain. Mutations in this gene may lead to carbohydrate-deficient glycoprotein syndrome, type II. Transcript variants with a spliced 5' uTR may exist, but their biological validity has not been determined. Source: Recombinant protein corresponding to aa30-447 from human MGAT2, fused to His-tag at N-terminus, expressed in E.coli. Molecular Weight: ~50kD (439aa) Applications: Suitable for use in SDS-PAGE. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.