MED27, Recombinant, Human, aa1-311, His-Tag (Mediator of RNA Polymerase II Transcription subunit 27, Mediator Complex Subunit 27, CRAP34, CRSP34, CRSP8, TRAP37)

Size

£576.00

SKU:: 218332
Molecular Weight:: 37.8
Purity:: ~80% (SDS-PAGE)
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 0.4M urea, 10% glycerol.
Extra Details:: MED27 is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. Source: Recombinant protein corresponding to aa1-311 from human MED27, fused to His-tag at N-terminus, expressed in E.coli. Molecular Weight: ~37.8kD (334aa) Applications: Suitable for use in SDS-PAGE. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.