LIME1, Recombinant, Human, aa28-295, His-Tag (Lck-Interacting Transmembrane Adapter 1 Precursor)

Size

£512.00

SKU:: 218308
Molecular Weight:: 30.8
Purity:: ≥80% (SDS-PAGE)
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 0.4M urea, 10% glycerol.
Extra Details:: Lck-interacting transmembrane adapter 1 precursor, also known as LIME1, was previously characterized as a transmembrane adaptor protein mediating TCR-dependent T cell activation. LIME1 becomes tyrosine phosphorylated after cross-linking of the CD4 or CD8 coreceptors. Phospho-LIME1 associates with the Src family kinase Lck and its negative regulator, Csk. Source: Recombinant protein corresponding to aa28-295 from human LIME1, fused to His-tag at N-terminus, expressed in E.coli. Molecular Weight: ~30.8kD (291aa) Applications: Suitable for use in SDS-PAGE. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.