JMJD6, Recombinant, Human, aa1-414, His-Tag (Bifunctional Arginine Demethylase and Lysyl-Hydroxylase JMJD6 Isoform 1)

Size

£576.00

SKU:: 218278
Molecular Weight:: 50
Purity:: ~90% (SDS-PAGE)
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a liquid in PBS, pH 7.4, 1mM DTT, 30% glycerol.
Extra Details:: JMJD6 is also known as bifunctional arginine demethylase and lysyl-hydroxylase JMJD6. JMJD6 acts as a lysyl-hydroxylase that catalyzes 5-hydroxylation on specific lysine residues of target proteins such as u2AF2/u2AF65 and LuC7L2, as a regulator of RNA splicing by mediating 5-hydroxylation of u2AF2/u2AF65, affecting the pre-mRNA splicing activity of u2AF2/u2AF65. In addition to peptidyl-lysine 5-dioxygenase activity, may act as an RNA hydroxylase, as suggested by its ability to bind single strand RNA. Also acts as an arginine demethylase which demethylates histone H3 at 'Arg-2' (H3R2me) and histone H4 at 'Arg-3' (H4R3me), thereby playing a role in histone code. Source: Recombinant protein corresponding to aa1-414 from human JMJD6, fused to His-tag at N-terminus, expressed in E.coli. Molecular Weight: ~50kD (437aa) Applications: Suitable for use in SDS-PAGE. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice