IL33, Recombinant, Mouse, aa109-266, His-Tag (Interleukin-33)

Size

£576.00

SKU:: 218260
Molecular Weight:: 18.1
Purity:: ~85% (SDS-PAGE)
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a liquid in PBS, 1mM DTT, 10% glycerol.
Extra Details:: Interleukin 33 (IL-33) is a 32kD proinflammatory cytokine that may also regulate gene transcription in producer cells. IL-33 is structurally related to IL-1, which induces helper T cells to produce type 2 cytokines and acts through the receptor IL1RL-1 (IL1 receptor-like-1), which is known also as ST2. Binding of IL-33 to this receptor activates NF-kappa-B and MAP kinases and induces in vitro Th2 cells to produce cytokines. In vivo, IL-33 induces expression of IL-4, IL-5, IL-13 and leads to severe pathological changes in mucosal organs and in vitro, it can be divided to N-terminal fragment of 12kD and C-terminal fragment of 18kD by cleavage of caspase-1. Source: Recombinant protein corresponding to aa109-266 from mouse IL33, fused to His-tag at N-terminus, expressed in E.coli. Molecular Weight: ~18.1kD (181aa), confirmed by MALDI-TOF Applications: Suitable for use in SDS-PAGE. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Endotoxin: <1.0Eu/1 microgram of protein (determined by LAL method) Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice