IL12, Recombinant, Human, aa23-328/aa23-219, His-Tag (Interleukin 12 (Subunit beta/alpha))

Size

£512.00

SKU:: 218253
Molecular Weight:: 34.640233
Purity:: ~85% (SDS-PAGE)
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a liquid in PBS, pH 7.4, 1mM EDTA, 20% glycerol.
Extra Details:: IL12 is a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. It is a disulfide-linked heterodimer composed of the 40kD cytokine receptor like subunit and a 35kD subunit. This cytokine is expressed by activated macrophages that serve as an essential inducer of Th1 cells development. IL12 has been found to be important for sustaining a sufficient number of memory/effector Th1 cells to mediate long-term protection to an intracellular pathogen. Source: Recombinant protein corresponding to human IL12, fused to His-tag at C-terminus, expressed in Insect cells. Molecular Weight: ~34.6kD (p40) & ~23.3kD (p35) (306aa & 203aa) The heterodimeric IL12 has a predicted molecular weight of ~57.9kD (509aa). Applications: Suitable for use in SDS-PAGE. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.