HMGN3, Recombinant, Human, aa1-77, His-Tag (High Mobility Group Nucleosomal Binding Domain 3)

Size

£512.00

SKU:: 218228
Molecular Weight:: 10.8
Purity:: ≥95% (SDS-PAGE)
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a liquid in 20mM Tris-HCl, pH 7.0, 10% glycerol.
Extra Details:: HMGN3, also known as high mobility group nucleosomal binding domain 3, binds thyroid hormone receptor beta, but only in the presence of thyroid hormone. Thyroid hormone receptors are hormone-dependent transcription factors that regulate expression of a variety of specific target genes. It is thought to reduce the compactness of the chromatin fiber in nucleosomes, thereby enhancing transcription from chromatin templates. Source: Recombinant protein corresponding to aa1-77 from human HMGN3, fused to His-tag at N-terminus, expressed in E.coli. Molecular Weight: ~10.8kD (100aa), confirmed by MALDI-TOF Applications: Suitable for use in SDS-PAGE. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.