Hepatitis C Virus NS5A, Recombinant, Hepatitis C Virus, aa2210-2306, His-Tag (HCV)

Size

£512.00

SKU:: 218217
Molecular Weight:: 13.4
Purity:: ≥90% (SDS-PAGE)
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 0.2M sodium chloride, 1mM DTT, 40% glycerol.
Extra Details:: NS5A is a component of the replication complex involved in RNA-binding. Its interaction with human VAPB may target the viral replication complex to vesicles. This protein down-regulates viral IRES translation initiation and mediates interferon resistance, presumably by interacting with and inhibiting human EIF2AK2/PKR. NS5a seems to inhibit apoptosis by interacting with BIN1 and FKBP8. The hyperphosphorylated form of NS5A is an inhibitor of viral replication. Source: Recombinant protein corresponding to aa2210-2306 from HCV (Hepatitis C Virus) NS5A, fused to His-tag at N-terminus, expressed in E.coli. Molecular Weight: ~13.4kD (118aa), confirmed by MALDI-TOF Applications: Suitable for use in SDS-PAGE. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.