218210
HADHB, Recombinant, Human, aa34-474, His-Tag (Hydroxyacyl Coenzyme A Dehydrogenase Beta, Trifunctional Enzyme Subunit beta, Mitochondrial)
Size
£512.00
- SKU:
- 218210
- Molecular Weight:
- 49.9
- Purity:
- ≥90% (SDS-PAGE)
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Format:
- Supplied as a liquid in 20mM phosphate, pH 8.0, 10% glycerol.
- Extra Details:
- HADHB is the beta subunit of the mitochondrial trifunctional protein, which catalyzes the last three steps of mitochondrial beta-oxidation of long chain fatty acids. The mitochondrial membrane-bound heterocomplex is composed of four alpha and four beta subunits, with the beta subunit catalyzing the 3-ketoacyl-CoA thiolase activity. This protein can also bind RNA and decreases the stability of some mRNAs. The genes of the alpha and beta subunits of the mitochondrial trifunctional protein are located adjacent to each other in the human genome in a head-to-head orientation. Mutations in this gene result in trifunctional protein deficiency. Source: Recombinant protein corresponding to aa34-474 from human HADHB, fused to His-tag at N-terminus, expressed in E.coli. Molecular Weight: ~49.9kD (464aa) Applications: Suitable for use in SDS-PAGE. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice