EEF1A1, Recombinant, Human, aa1-462 (Eukaryotic Translation Elongation Factor 1 Alpha 1, CCS-3, CCS3, EEF-1, EEF1A, eEF1A-1, EF-Tu, EF1A, FLJ25721, GRAF-1EF, HNGC:16303, PTI1)

Size

£512.00

SKU:: 218122
Molecular Weight:: 50
Purity:: ≥90% (SDS-PAGE)
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 0.4M urea, 10% glycerol.
Extra Details:: EEF1A1 is an isoform of the alpha subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This isoform (alpha 1) is expressed in brain, placenta, lung, liver, kidney, and pancreas, and the other isoform (alpha 2) is expressed in brain, heart and skeletal muscle. This isoform is identified as an autoantigen in 66% of patients with Felty syndrome. This gene has been found to have multiple copies on many chromosomes, some of which, if not all, represent different pseudogenes. Source: Recombinant protein corresponding to aa1-462 from human EEF1A1 expressed in E.coli. Molecular Weight: ~50kD (462aa) Applications: Suitable for use in SDS-PAGE. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.