CGREF1, Recombinant, Human, aa20-301, His-Tag (Cell Growth Regulator With EF-Hand Domain 1)

Size

£576.00

SKU:: 218030
Molecular Weight:: 32.3
Purity:: ≥85% (SDS-PAGE)
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a liquid in PBS, pH 7.4. No preservative added.
Extra Details:: CGREF1, also known as cell growth regulator with EF-hand domain 1, is a secreted calcium ion binding protein. This protein contains two EF-hand domains and both EF-hands are required for function. It is probably digested extracellularly by an unknown serine protease generating extremely hydrophobic bioactive peptides. It mediates cell-cell adhesion in a calcium-dependent manner. In addition, CGREF1 is able to inhibit growth in several cell lines. Recombinant protein corresponding to aa20-301 from human CGREF1, fused to His-tag, expressed in E. coli. Uniprot/Accession: AAH34764 Molecular Weight: ~32.3kD (305aa), confirmed by MALDI-TOF Applications: Suitable for use in SDS-PAGE. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSAPKDGVT RPDSEVQHQL LPNPFQPGQE QLGLLQSYLK GLGRTEVQLE HLSREQVLLY LFALHDYDQS GQLDGLELLS MLTAALAPGA ANSPTTNPVI LIVDKVLETQ DLNGDGLMTP AELINFPGVA LRHVEPGEPL APSPQEPQAV GRQSLLAKSP LRQETQEAPG PREEAKGQVE ARRESLDPVQ EPGGQAEADG DVPGPRGEAE GQAEAKGDAP GPRGEAGGQA EAEGDAPGPR GEAGGQAEAR ENGEEAKELP GETLESKNTQ NDFEVHIVQV ENDEI Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice