CA14, Recombinant, Human, aa16-290, His-Tag (Carbonic Anhydrase 14)

Size

£512.00

SKU:: 217987
Molecular Weight:: 33.2
Purity:: ≥85% (SDS-PAGE)
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 0.15M sodium chloride, 1mM DTT, 10% glycerol.
Extra Details:: Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA14 is predicted to be a type I membrane protein and shares highest sequence similarity with the other transmembrane CA isoform, CA XII; however, they have different patterns of tissue-specific expression and thus may play different physiologic roles. Source: Recombinant corresponding to aa16-290 from human CA14 protein, fused to His-tag at N-terminus, expressed in E.coli. Molecular Weight: ~33.2kD (298aa), confirmed by MALDI-TOF Enzyme Activity: Specific activity is >700 pmol/min/ug, and is defined as the amount of enzyme that hydrolyzes 1.0 pmole of 4-nitrophenyl acetate to 4-nitrophenol per minute at pH 7.5 and 37°C. Applications: Suitable for use in SDS-PAGE. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice