Akr1b1, Recombinant, Mouse, aa1-316, His-Tag (Aldose Reductase)

Size

£512.00

SKU:: 217934
Molecular Weight:: 38.1
Purity:: ≥95% (SDS-PAGE)
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a liquid in PBS, pH 7.4, 10% glycerol.
Extra Details:: Akr1b1, also known as Aldose reductase, is an enzyme. It catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols with a broad range of catalytic efficiencies. Source: Recombinant protein corresponding to aa1-316 from mouse Akr1b1, fused to His-tag at N-terminus, expressed in E.coli. Molecular Weight: ~38.1kD (339aa), confirmed by MALDI-TOF Enzyme Activity: Specific activity is >500 pmol/min/ug, and is defined as the amount of enzyme that catalyzes the reduction of 1.0 pmole DL-glyceraldehyde in the presence of NADPH per minute at pH 7.0 and 37°C. Applications: Suitable for use in SDS-PAGE. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.