ACAD8, Recombinant, Human, aa23-415, His-Tag (Isobutyryl-CoA Dehydrogenase, Mitochondrial)

Size

£512.00

SKU:: 217919
Molecular Weight:: 45.1
Purity:: ≥95% (SDS-PAGE)
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 0.15M sodium chloride, 1mM DTT, 30% glycerol.
Extra Details:: ACAD8 is a member of the acyl-CoA dehydrogenase family of enzymes that catalyze the dehydrogenation of acyl-CoA derivatives in the metabolism of fatty acids or branch chained amino acids. The protein is a mitochondrial enzyme that functions in catabolism of the branched-chain amino acid valine. Defects in this gene are the cause of isobutyryl-CoA dehydrogenase deficiency. Source: Recombinant protein corresponding to aa23-415 from human ACAD8, fused to His-tag at N-terminus, expressed in E.coli. Molecular Weight: ~45.1kD (416aa), confirmed by MALDI-TOF Enzyme Activity: Not determined. This product is recommended for use in applications that do not require a catalytically active form of the protein. Applications: Suitable for use in SDS-PAGE. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.