Cytochrome P450 26A1 (Retinoic acid-metabolizing cytochrome, P450 retinoic acid-inactivating 1, P450RAI, hP450RAI, Retinoic acid 4-hydroxylase, CYP26A1)

Size

£512.00

SKU:: 217892
Purity:: Supernatant
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Ovalbumin-conjugated synthetic peptide PARFTHFHGE (C-terminal sequence)
Clone:: F27 P6 A1
Format:: Supplied as a liquid.
Extra Details:: CYP26A1 is significantly overexpressed in colorectal cancer Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: ELISA: Neat Western Blot: 1:5 Immunohistochemistry: Neat. Antigen retrieval: microwave 20 min @ 800W in 10mM citrate buffer, pH 6.0 Optimal dilutions to be determined by the researcher. Positive Control: Immunohistochemistry: formalin-fixed, paraffin-embedded liposarcoma sections. Western Blot: recombinant P450, 18.2 kDa c-terminus region. Western Blot: over expression lysates Hybridoma: Ag8.653 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.