Cytochrome P450 1B1 (CYP1B1)

Size

£512.00

SKU:: 217891
Purity:: Supernatant
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Ovalbumin-conjugated synthetic peptide corresponding to aa437-451, PENFDPARFLDKDGL.
Clone:: 5D3
Format:: Supplied as a liquid.
Extra Details:: CYP1B1 was detected only in cancer tissues and is considered a universal tumor marker. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:10 - 1:100 Immunohistochemistry: 1:5. Antigen retrieval: microwave 20 min @ 800W in 10mM citrate buffer, pH 6.0 Optimal dilutions to be determined by the researcher. Positive Control: Immunohistochemistry: formalin-fixed, paraffin-embedded ovarian cancer sections Western Blot: rhCYP1B1 from lymphoblastoid cells (Gentest); 10ug microsomal protein/lane Hybridoma: Ag8.653 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.