Siglec-9 (Sialic Acid Binding Ig-like Lectin 9, CD329, OBBP-Like)

Size

£889.00

SKU:: 217885
Purity:: Purified by Protein A affinity chromatography from hybridoma culture supernatant. Endotoxin: ≤1EU/ug (LAL).
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to Gln18-Gly348 (predicted) of human Siglec-9, expressed in NS0 cells.
Clone:: 7H6
Format:: Supplied as a lyophilized powder in PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
Extra Details:: Siglecs are I-type (Ig-type) lectins belonging to the Ig superfamily. They are characterized by an N-terminal, Ig-like V-type domain that mediates sialic acid binding, followed by varying numbers of Ig-like C2-type domains (2 to 17), a single transmembrane region, and a cytoplasmic tail. The siglecs can be broadly classified into two subgroups: Siglecs-1, -2, and -4, and a Siglec-3/CD33-related subgroup (Siglecs-3, and -5 through -13 in primates) defined by sequence similarity and clustered gene localization. They are widely expressed on hematopoietic cells, often in a cell-type-specific manner, and Siglec-4/MAG is a myelin component in Schwann cells and oligodendrocytes. Their ligands, sialic acids, are negatively charged monosaccharides found on cell-surface glycoproteins and glycolipids. Although Siglec functions continue to be defined, most have intracellular immunoreceptor tyrosine-based inhibitory motifs (ITIM), implicating them in the suppression of immunoreceptor signaling. They may also participate in cell/cell interactions or act as receptors for the entry of viral or bacterial pathogens. Applications: Suitable for use in Flow Cytometry, ELISA, Western Blot and Neutralization. Other applications not tested. Recommended Dilutions: Flow Cytometry: 0.25ug/10e6 cells using human peripheral blood granuloytes ELISA (capture): 2-8ug/ml Western Blot: 1ug/ml using recombinant human Siglle-9 Fc Chimera under reducing conditions only Neutralization: Measured by its ability to neutralize Siglec-9-mediated adhesion of human RBCs. (Kelm, S. et al. (1994) Current Biology 4:965). The Neutralization Dose (ND50) is typically 0.015-0.075ug/ml in the presence of 10ug/ml recombinant human Siglec-9 Fc Chimera. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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