Tumor Necrosis Factor, Recombinant, Human

Size

£774.00

SKU:: 217858
Purity:: ≥97% (SDS-PAGE)
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a lyophilized powder in 3mM Tris, pH 8.0. Reconstitute with 50ul sterile ddH2O.
Extra Details:: Tumor Necrosis Factor-alpha (TNF-alpha) is a potent lymphoid factor that exerts cytotoxic effects on a wide range of tumor cells and certain other target cells. The mature form of human TNF-alpha has 157aa residues. 217858 is a recombinant polypeptide containing the mature form of human TNF-a with a polyhistidine tag at its N-terminal domain. Recombinant protein corresponding to human tumor necrosis factor-a expressed in E. coli. Uni/Swiss Prot Number: P01375 Biological Activity: Fully biologically active when compared to standards. The ED50 as determined by the cytolysis of mouse L929 cells in the presence of Actinomycin-D is <= 0.05ng/ml. Specific Activity: >2x10e7 units/mg Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Allow the reconstituted vial to sit at room temperature for 2 hours before use. This solution can be diluted into other buffered solutions and stored at 4°C for one week or -20°C for future use.