Tumor Necrosis Factor (TNF, TNF-alpha, TNFA, TNF-a, Cachectin, DIF, Tumor Necrosis Factor Ligand Superfamily Member 2, TNFSF2)

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£698.00

SKU:: 217856
Purity:: Purified by Protein A affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to human tumor necrosis factor a, expressed in E. coli.
Clone:: 2C8
Format:: Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Extra Details:: Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Applications: Suitable for use in ELISA, Immunohistochemistry and Neutralization. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. TNF-α Neutralization Assay: 1. Plate 2x10e4 mouse L929B cells into individual wells of 96-well (flat bottomed) microtiter plate. 2. Incubate overnight at 37°C in humidified 5% CO2 incubator. 3. The next day, prepare serial dilutions of anti-TNF (human) in medium containing 2ug/mL of actinomycin D, for a final volume of 50ul 4. Add 50uL of TNF (10ng/mL final concentration) to each antibody dilution and incubate for 1 hour at 37°C. 5. Add 100uL of the antibody/antigen mixture to the pre-seeded L929B cells. 6. Incubate 24 hours at 37°C, 5% CO2. 7. Score wells for cytotoxicity. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice