Tumor Necrosis Factor (TNF, TNF-alpha, TNFA, TNF-a, Cachectin, DIF, Tumor Necrosis Factor Ligand Superfamily Member 2, TNFSF2)

Size

£754.00

SKU:: 217855
Purity:: Purified.
Storage Conditions:: 4°C Do Not Freeze
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to human tumor necrosis factor-alpha expressed in E. coli.
Clone:: 195
Format:: Supplied as a liquid in 0.01M PBS, pH 7.1, 0.15M sodium chloride.
Extra Details:: Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Applications: Suitable for use in Western Blot, Immunohistochemistry, Immunocytochemistry and Neutralization. Other applications not tested. Recommended Dilution: Neutralization: 50ng of TNF-alpha antibody is required for the neutralization of 1U TNF-alpha. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.