TNFB (TNF-beta, Tumor Necrosis Factor Ligand Superfamily Member 1, LTA, TNFSF1, LT-alpha, Lymphotoxin-alpha)

Size

£735.00

SKU:: 217842
Purity:: Purified by Protein A affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to human TNFB expressed in E. coli.
Clone:: 9B9
Format:: Supplied as a lyophilized powder from PBS, 20ug raffinose. Reconstitute with 1ml sterile dH2O.
Extra Details:: Cytokine that in its homotrimeric form binds to TNFRSF1A/TNFR1, TNFRSF1B/TNFBR and TNFRSF14/HVEM. In its heterotrimeric form with LTB binds to TNFRSF3/LTBR. Lymphotoxin is produced by lymphocytes and cytotoxic for a wide range of tumor cells in vitro and in vivo. Applications: Suitable for use in Western Blot, Immunohistochemistry, Immunocytochemistry and Neutralization. Other applications not tested. Recommended Dilutions: Neutralization: 200ng of TNF-beta antibody is required for the neutralizaion of 1unit/ml TNF-beta. The TNF-beta-preparation which is used has a specific activity of ca. 2x10e7units/mg protein [cell lytic assay with L 929 cells (mouse transformed fibroblasts) and/or WEHJ 164 cells (mouse fibrosarcoma cells)/actinomycin D]. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice