217839
TMEM16A Activator, Eact (3,4,5-trimethoxy-N-(2-methoxyethyl)-N-(4-phenylthiazol-2-yl)benzamide, ANO1 Activator)
Size
£372.00
- SKU:
- 217839
- Molecular Weight:
- 428.5
- Purity:
- ~98% (HPLC)
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Format:
- Supplied as a white powder.
- Extra Details:
- An N-aroylaminothiazole that strongly activates TMEM16A (ANO1) calcium-activated chloride channel (CaCC) at 0 Ca2+ concentration (Ca2+ independent), with the capability of producing large and sustained CaCC Cl-1 currents (EC50=3uM) in TMEM16A-expressing Fisher Rat Thyroid (FRT) Cells without elevation of cytoplasmic Ca2+ level, and in human A253 cells. Shown to increase CaCC conductance in human salivary and airway submucosal gland epithelial cells, IL-4 treated bronchial cells, and stimulated submucosal gland secretion in human bronchi and smooth muscle contraction in mouse intestine. Activation effect can be abolished by the addition of a TMEM16A inhibitor T16A-A01 at 10uM in TMEM16A-transfected cells and in cultures of human salivary gland and IL-4-treated bronchial epithelia, but not in intestine. Does not affect CFTR Cl- conductance or ENaC Na+ conductance. Formula: C22H24N2O5S Molecular Weight: 428.5 Solubility: DMSO (100mg/ml, clear, colorless solution) Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile buffer or dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice