PME-1 Inhibitor, AMZ-30

Size

£386.00

SKU:: 217678
Molecular Weight:: 462.6
Purity:: ≥97% (HPLC)
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a yellow solid.
Extra Details:: A cell-permeable sulfonyl acrylonitrile compound that acts as a potent and irreversible inhibitor of protein phosphatase methylesterase-1 (PME-1; IC50=600nM and 2.5uM in human cell lysates and in HEK 293T cells, (respectively) with 100-fold greater selectivity over other serine hydrolases; does not function as a general thiol-reactive probe. Shown to lower demethylated PP2A levels by ~80% at 20uM with elevated methylated PP2A (protein phosphatase 2A) form in PME-1 overexpressing HEK 293T cells. Synonyms: (E)-2-(4-Fluorophenylsulfonyl)-3-(1-(3-nitrophenylsulfonyl)-1H-pyrrol-2-yl)-acrylonitrile, NIH Probe ML136 Molecular Formula: C₁₉H₁₃FN₃O₆S₂ Molecular Weight: 462.6 Solubility: DMSO (50mg/ml) Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile buffer or dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.