Phosphodiesterase 9A (PDE9A, High Affinity cGMP-specific 3',5'-cyclic Phosphodiesterase 9A, FLJ90181, HSPDE9A2)

Size

£825.00

SKU:: 217653
Purity:: Purified by Protein A affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Linear peptide corresponding to human PDE9A, conjugated to KLH. Species sequence homology: rat and chimpanzee (100%)
Format:: Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
Extra Details:: PDE9A is a ubiquitous protein belonging to the cyclic nucleotide phosphodiesterase family which regulate the signaling of cyclic nucleotides such as cGMP and cAMP. PDE9A has a higher affinity for cGMP. It contains a catalytic site that associates with cofactors and hydrolyzes cGMP thereby terminating cGMP signaling for a number of cellular processes. PDE9A has been indirectly linked to signaling pathways involved in cognitive processes. Previous studies suggested that inhibition of PDE9A enhanced long-term potentiation (a widely studied mechanism of learning and memory); PDE9A may also effect changes in the plasticity of hippocampal neurons which are also important for certain types of cognitive processes. In addition to the brain, PDE9A is also highly expressed in the spleen, prostate, colon, kidney, and heart and may be involved in a range of physiological processes in these systems, via cGMP-amplified signaling. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry: 1:100 detected PDE9 in human prostate tissue, and in possible golgi type II and basket cells in the Purkinje and molecular layers of mouse cerebellum. Optimal dilutions to be determined by the researcher. Control: Human spleen tissue lysate Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice