PDE11A (Dual 3',5'-cyclic-AMP and -GMP Phosphodiesterase 11A, cAMP and cGMP Phosphodiesterase 11A)

Size

£800.00

SKU:: 217628
Purity:: Purified by affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: KLH-conjugated linear peptide corresponding to the GAF domain of human PDE11A. Species sequence homology: rat and bovine (100%)
Format:: Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
Extra Details:: The PDE11A gene encodes for a dual-specificity Phosphodiesterase (PDE), which is expressed in the adrenal cortex and is partially inhibited by tadalafil and other PDE inhibitors. PDEs are known to catalyze cAMP and cGMP to 5'-AMP and 5'GMP, respectively and thereby regulate the intracellular concentration of these nucleotides. Loss-of-function mutation of the PDE11A gene is shown to increase cAMP signaling and cAMP-responsive element binding protein (CREB) phosphorylation, two conditions which are associated with adrenocortical hyperplasia, adenomas, and Cushing syndrome. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry: 1:1000 detected PDE11A in normal mouse midbrain tissue. Western Blot: 1ug/ml detected PDE11A in 10ug of human hippocampus tissue lysate. Optimal dilutions to be determined by the researcher. Control: Human hippocampus tissue lysate Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice