MET (Hepatocyte Growth Factor Receptor, HGF Receptor, HGF/SF Receptor, Proto-oncogene c-Met, Scatter Factor Receptor, SF Receptor, Tyrosine-protein Kinase Met)

Size

£819.00

SKU:: 217543
Purity:: Purified.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to human HGF.
Clone:: 4C12.1
Format:: Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide.
Extra Details:: Hepatocyte Growth Factor (HGF), also known as Scatter Factor (SF) and Hepatopoietin A, is a pleiotrophic growth factor which is produced by mesodermally derived cells such as endothelial cells, Kupffer cells/macrophages, and hepatic fat-storing cells. HGF/SF is a potent effector of growth, movement, differentiation and invasiveness of epithelial and cancer cells. It can induce epithelial-mesenchymal conversion of epithelial cells in culture, support epithelial branching duct formation in the developing lung and elicit a potent organotrophic function for regeneration of liver, kidney and lung through epithelial-stromal interactions. HGF/SF is also a potent angiogenic molecule whose activity such as cell migration, proliferation, protease production, invasion and capillary tube formation is mediated through direct actions on vascular endothelial cells. Recent evidence has shown that HGF/SF is overexpressed in invasive human cancer, relative to non-invasive and benign conditions. In invasive breast cancers, the expression of HGF/SF is strongly correlated with von Willebrand Factor, a marker of endothelial cells. The invasive and metastatic signals given by HGF/SF are transduced through the c-met proto-oncogene product, a transmembrane tyrosine kinase that functions as the HGF/SF receptor. Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 1:200-1:400 Immunoprecipitation: 10ul/mg protein lysate Optimal dilutions to be determined by the researcher. Positive Control: Endothelial cells, Kupffer cells, or macrophages Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice