LIM Kinase Inhibitor I, LIMKi 3 (N-(5-(1-(2,6-Dichlorophenyl)-3-(difluoromethyl)-1H-pyrazol-5-yl)thiazol-2-yl)isobutyramide)

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£594.00

SKU:: 217513
Molecular Weight:: 431.3
Storage Conditions:: 4°C
Supplier:: United States Biological
CAS Number:: 1338247-35-0
Extra Details:: A cell-permeable pyrazolylthiazolo-isobutyramide compound that acts as a potent LIM kinase inhibitor (IC50 = 7 and 8nM against LIMK1 and LIMK2, respectively) and effectively suppresses cellular cofilin phosphorylation (IC50 ~1uM in A549 and MDA-MB-231 cultures) without affecting tubulin polymerization or inducing cytotoxicity (EC50 >10uM in A549 proliferation & colony formation assays). Shown to effectively destabilize F-actin structure in MDA-MB-231 breast cancer cells (3 to 10uM) with concomitant blockage of invasion (by 93% at 10uM). Synonyms: N-(5-(1-(2,6-Dichlorophenyl)-3-(difluoromethyl)-1H-pyrazol-5-yl)thiazol-2-yl)isobutyramide CAS No: 1338247-35-0 Molecular Formula: C₁₇H₁₄Cl₂F₂N₄OS Solubility: DMSO Primary Target: LIMK1 and LIMK2 Primary Target IC50: 7 and 8nM against LIMK1 and LIMK2, respectively Secondary Target: AMPKA1, AMPKA2, DDR1, PAK3, DCAMKL2 Molar Mass: 431.3 Purity: ≥95% (HPLC) Form: Supplied as a beige powder. Storage and Stability: Store at 4°C under inert atmosphere. For maximum recovery of product, centrifuge the original vial prior to removing the cap.