JAK2, phosphorylated (Tyr570) (Tyrosine-protein Kinase JAK2, Janus Kinase 2, JAK-2)

Size

£732.00

SKU:: 217477
Application:: WB
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Linear peptide corresponding to human JAK-2 phosphorylated at Tyr570, conjugated to KLH.
Format:: Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
Extra Details:: JAK-2 (Janus Kinase 2) belongs to the emerging family of non-receptor Janus tyrosine kinases, which regulate a spectrum of cellular functions downstream of activated cytokine receptors in the lympho-hematopoietic system. Immunological stimuli, such as interferons and cytokines, induce recruitment of Stat transcription factors to cytokine receptor-associated JAK-2. JAK-2 then phosphorylates proximal Stat factors, which subsequently dimerize, translocate to the nucleus and bind to CIS elements upstream of target gene promoters to regulate transcription. The canonical JAK/Stat pathway is integral to maintaining a normal immune system by stimulating proliferation, differentiation, survival and host resistance to pathogens. Altering JAK/Stat signaling to reduce cytokine induced pro-inflammatory responses represents an attractive target for anti-inflammatory therapies. Within the JAK-2 kinase domain, there is a region that has considerable sequence homology to the regulatory region of the insulin receptor. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 0.05ug/ml detects JAK-2 on 10ug of transfected HEK293T cell lysates. Optimal dilutions to be determined by the researcher. Control: Transfected HEK293T cell lysates Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice