Histone Lysine Demethylase Inhibitor VIII, GSK-J4

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£462.00

SKU:: 217431
Molecular Weight:: 417.5
Purity:: ≥98% (HPLC)
Storage Conditions:: 4°C
Supplier:: United States Biological
CAS Number:: 1373423-53-0
Format:: Tan semi-solid
Extra Details:: A cell-permeable GSK-J1 prodrug that is ineffective in cell-free assays, but is effectively converted to GSK-J1 intracellularly for effective JMJD3 and UTX inhibition (effective conc. 25 to 50uM in HeLa cultures). GSK-J4 treatment is shown to block LPS-induced TNF-alpha production in primary human macrophage cultures (IC50 = 9uM by ELISA). GSK-J4 has been used to study the effect of KDM2B (Jumonji (JmjC) domain histone 3 lysine 36 (H3K36) di-demethylase) inhibition on the survival and DNA repair potential of glioblastoma cells. It has also been used in sulforhodamine B (SRB) cell growth assay and cell viability assay. Synonyms: GSK-J1 Pro-Drug, JHDM Inhibitor II Pro-Drug, Ethyl-3-(6-(4,5-dihydro-1H-benzo[d]azepin-3(2H)-yl)-2-(pyridin-2-yl)pyrimidin-4-ylamino)propanoate CAS No: 1373423-53-0 Molecular Formula: C₂₄H₂₇N₅O₂ Molecular Weight: 417.5 Appearance: Tan semi-solid Purity: ≥98% (HPLC) CAS No: 1373423-53-0 Primary Target: H3K27me3 demethylases JMJD3 & UTX Solubility: DMSO Storage and Stability: May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.