H-89 Dihydrochloride (PKA Inhibitor III, N-[2-((p-Bromocinnamyl)amino)ethyl]-5-isoquinolinesulfonamide, 2HCl)

Size

£344.00

SKU:: 217411
Molecular Weight:: 519.3
Purity:: ~99% (HPLC)
Storage Conditions:: -20°C
Supplier:: United States Biological
CAS Number:: 127243-85-0
Format:: Supplied as an off-white solid.
Extra Details:: A cell-permeable, selective, reversible, ATP-competitive and potent inhibitor of protein kinase A (Ki = 48nM). Inhibits other kinases only at much higher concentrations: CaM kinase II (Ki = 29.7uM), casein kinase I (Ki = 38.3uM), myosin light chain kinase (Ki = 28.3uM), protein kinase C (Ki = 31.7uM), and ROCK-II (IC50 = 270nM). May be used to discriminate between the effects of PKA and cAMP-regulated guanine-nucleotide-exchange factors (GEFs) such as GEFI, GEFII, or Epac (exchange protein directly activated by cAMP). Enhances radiation-induced apoptosis in the human cell line BM 13674. Formula: C20H20BrN3O2S · 2HCl Solubility: DMSO (200mg/ml), H2O (25mg/ml) Molecular Weight: 519.3 Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile buffer or dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.