DUB Inhibitor VI, P22077 (1-(5-(2,4-Difluorophenylthio)-4-nitrothiophen-2-yl)ethanone, USP7 Inhibitor II, USP47 Inhibitor II)

Size

£429.00

SKU:: 217330
Molecular Weight:: 315.3
Purity:: ≥98% (HPLC)
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as an off-white solid.
Extra Details:: A cell-permeable thiophenylethanone that is shown to inhibit USP7 and USP47 deubiquitinase activity (IC50=8.01 & 8.74uM, respectively), while exhibiting much reduced affinity toward ATXN3, BAP1, and USP1, and displaying no inhibitory activity toward other known PR-619 targets (IC50 >50uM). Both P22077 and PR-619 are shown to increase overall protein polyubiquitination in HEK293T cells in a dose- and time-dependent manner (20 to 150uM; 0.5 to 20h), however PR619 treatment results in upregulation of both K48- and K63-linked, while P22077 exposure results in mainly enrichment of K48-linked polyUb chains. Formula: C12H7F2NO3S2 Molecular Weight: 315.3 Solubility: DMSO (50mg/ml) Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile buffer or dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.