217240
Calmodulin Binding Protein Epitope Tag (CBP-Tag)
Size
£960.00
- SKU:
- 217240
- Application:
- WB
- Purity:
- Supernatant
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide corresponding to the Calmodulin Binding Protein (CBP) epitope tag found in several E. coli expression vectors, conjugated to KLH.
- Clone:
- C16T
- Format:
- Supplied as a liquid in 0.05% sodium azide.
- Extra Details:
- Calmodulin Binding Protein (CBP) epitope tags are found in several E. Coli expression vectors. Calmodulin (CaM), a small 17kD ubiquitous Ca2+ binding protein, acts as an intracellular Ca2+ receptor and transduces Ca2+ transients, usually in response to growth factors. It mediates the activity of a number of Ca2+ regulating enzymes, including protein kinases, phosphatases, nitric oxide synthases, and phosphodiesterase. Calmodulin is the principal intracellular calcium receptor. It is an acidic protein of 148 residues, and upon binding Ca2+, it in turn binds to short peptide sequences of many target proteins. Such interaction is thought to induce a conformational change on the target, resulting in its activation. Targets include the constitutively expressed nitric oxide synthases, the CaM kinases, and PP2B/Calcineurin. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:2000 detected a recombinant protein containing the calmodulin binding protein epitope tag in lysates from transformed E. coli. Optimal dilutions to be determined by the researcher. Control: Samples containing the calmodulin binding protein epitope tag. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice