Berberine Chloride (Berberine)

Size

£236.00

SKU:: 217216
Molecular Weight:: 371.8
Purity:: ~95% (HPLC) (NMR if no UV signal)
Storage Conditions:: -20°C
Supplier:: United States Biological
CAS Number:: 633-65-8
Format:: Supplied as an off-white solid.
Extra Details:: A highly potent and selective oxysterol EBI2 (GPR183) agonist (Kd) = 450pM in a saturation binding assay, and EC50 = 140 pM over EC50 = 2.1nM for its enantiomer, 7b,25-OHC, in a GTP-gamma S binding assay). Dose-dependently suppresses forskolin-induced cAMP accumulation in an EBI2-expressing SK-N-MC/CRE-b-galactosidase cell line (IC50 = 2nM), but not in control cells. Stimulates migration of LPS-activated spleen B-cells and anti-CD3/CD28-activated CD4+ T-cells in a dose-dependent manner. In addition, pharmacological inhibition of its biosynthesis in vivo by Clotrimazole, a CYP7B1inhibitor, promotes the migration of adoptively transferred pre-activated B cells to the T/B boundary, mimicking the phenotype of pre-activated B cells in EBI2-deficient mice. CAS Number: 633-65-8 Formula: C₂₀H₁₈NO₄+Cl- Molecular Weight: 371.8 Solubility: DMSO (10mg/ml) or H2O (1mg/ml) Storage and Stability: May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.