7-alpha, 25-dihydroxycholesterol (EBI2 agonist, EBI2 Activator, GPR183 Activator, 7alpha,25-OHC)

Size

£567.00

SKU:: 217131
Purity:: ~98% (HPLC)
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a white powder.
Extra Details:: A highly potent and specific oxysterol EBI2 (GPR183) agonist with a kd of 450pM in a saturation binding analysis, and activates EBI2 (EC50=140pM compared with 2.1nM for its enantiomer 7b,25-OHC) in a GTP-gamma assay. Dose-dependenty suppresses forskolin-induced cAMP accumulation in EBI2-expressing SK-N-MC/CRE-beta galactosidase cell line with an IC50 of 2nM, but not in control cells. Stimulates migration of LPS-activated spleen B cells and T cells in a dose-dependent manner. In addition, pharmacological inhibition of its biosynthesis in vivo by Clotrimazole, a CYP7B1inhibitor promotes the migration of adoptively transferred pre-activated B cells to the T/B boundary, mimicking the phenotype of pre-activated B cells from EBI2-deficient mice. Formula: C₂₇H₄₆O₃ Solubility: DMSO Molar Mass: 418.3 Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.