WNT-3a (WNT3A)

Size

£691.00

SKU:: 217108
Purity:: Purified by Protein G affinity chromatography from culture supernatant.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rat
Immunogen:: Recombinant protein corresponding to mouse Wnt-3a.
Clone:: 14L1008
Format:: Supplied as a lyophilized powder in PBS. Reconstitute with 200ul sterile PBS.
Extra Details:: WNT-3a, is a secreted protein and member of the Wnt family of signalling proteins, which acts as a ligand for members of the frizzled family of seven transmembrane receptors, and plays key roles in many developmental and cell fate decisions, including embryonic patterning, CNS development, and cytoskeletal formation. Wnt-3a acts as a promoter of the proliferation of ventral midbrain dopaminergic neuron precursor cells, and has been implicated in the progressive differentiation of anterior neural cells, as well as regulating the survival and expansion of neural progenitors derived from human embryonic stem cells. Wnt3a has been shown to act upstream of the Notch signalling pathway, in the regulation of somitogenesis, which is believed to be controlled by a segmentation clock, consisting of molecular oscillators in the Notch, Wnt3a and Fgf8 pathways. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:250-1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice