VEGF-A (Vascular Endothelial Growth Factor A)

Size

£704.00

SKU:: 217076
Purity:: Purified by Protein A/G affinity chromatography
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant human VEGF-A 165 protein
Clone:: 14L979
Format:: Supplied as a lyophilized powder in PBS. Reconstitute with 500ul sterile PBS.
Extra Details:: VEGFA was originally isolated as a heparin-binding secreted factor from bovine pituitary folliculo-stellate cells. It is a direct acting mitogen specific for vascular endothelial cells and capable of stimulating angiogenesis in vivo. In addition to inducing endothelial cell proliferation it promotes cell migration, inhibits apoptosis, and induces permeabilization of blood vessels. Molecular cloning has revealed multiple forms of VEGFA arising from alternative splicing of the RNA transcript. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:400-1600 Immunohistochemistry: 1:25-50 Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.