Tumor Necrosis Factor alpha (TNFa, TNF alpha)

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£691.00

SKU:: 217059
Purity:: Purified by Protein G affinity chromatography from culture supernatant.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to human TNF alpha
Clone:: 14L962
Format:: Supplied as a lyophilized powder in PBS. Reconstitute with 200ul sterile PBS.
Extra Details:: TNF-a is secreted by macrophages, monocytes, neutrophils, T-cells (principally CD4+), and NK-cells. Many transformed cell lines also secrete TNF-a. Monomeric mouse TNF-a is 156 amino acid protein (N-glycosylated) with a reported molecular weight of 17.5kD protein. TNF-a forms multimeric complexes; stable trimers are most common in solution. A 26kD membrane form of TNF-a has also been described. TNF-a binding to surface receptors elicits a wide array of biologic activities including: cytolysis and cytostasis of many tumor cell lines in vitro, hemorraghic necrosis of tumors in vivo, increased fibroblast proliferation, and enhanced chemotaxis and phagocytosis in neutrophils. Applications: Suitable for use in Neutralization, Western Blot, and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:500-1000 Immunohistochemistry: 1:100-200 Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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