TGFB1 (Transforming Growth Factor beta-1, TGF-beta-1, TGFB, LAP)

Size

£704.00

SKU:: 216985
Purity:: Purified by Protein G affinity chromatography from culture supernatant.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to human LAP TGF beta 1
Clone:: 14L888
Format:: Supplied as a lyophilized powder in PBS. Reconstitute with 200ul sterile PBS.
Extra Details:: TGF-B1 (transforming growth factor B1) is a multifunctional cytokine which regulates cellular proliferation and differentiation. It is ubiquitously expressed by many types of cells. Platelets express high level of TGF-B. TGF-B is synthesized as a large protein precursor and then secreted as a complex of TGF-B and LAP (latency-associated peptide), in which LAP noncovalently associates with the dimeric mature TGF-B to prevent its activity. TGF-B requires activation before it binds to its receptors and exerts functions. It has been reported that LAP-TGF-B binds to the integrins avB1, avB6, avB8, and a8B1 through RGD domain. TGF-B plays important roles in controlling proliferation and differentiation of epithelial cells, endothelial cells, fibroblasts, neurons, osteoclasts, and osteoblasts. TGF-B is believed to be important in the regulation of the development of Treg, Th17, and Th9 cells. A recent study has shown that LAP is an activated Treg surface marker. Applications: Suitable for use in Neutralization and Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:500-1000 Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice