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216878

PCPE-1 (PCOLE1)

Size

£704.00

SKU:
216878
Application:
WB
Purity:
Purified by Protein G affinity chromatography from culture supernatant.
Storage Conditions:
-20°C
Supplier:
United States Biological
Host:
Rat
Immunogen:
Recombinant protein corresponding to mouse PCPE-1.
Clone:
14L782
Format:
Supplied as a lyophilized powder in PBS. Reconstitute with 200ul sterile PBS.
Extra Details:
PCOLE1 (PCPE-1) is a secreted 55kD enzyme adaptor that enhances the activity of the BMP-1/tolloid family of metalloproteinases (also known as procollagen C-proteinases) on fibrillar procollagens. PCOLE1 binds to the C-propeptide region and also elsewhere in the procollagen molecule to specifically stimulate BMP-1/tolloid processing of procollagen I, II, and III. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:100-1:500 Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Shipping Conditions:
Blue Ice