MMP-13 (MMP13, Matrix Metalloproteinase 13)

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£704.00

SKU:: 216829
Purity:: Purified by Protein G affinity chromatography from culture supernatant.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to human MMP-13
Clone:: 14L733
Format:: Supplied as a lyophilized powder in PBS. Reconstitute with 500ul sterile PBS.
Extra Details:: MMP13 is a Metallopeptidase M10A type protease. Metalloproteinase 13/Collagenase 3 (MMP13) was originally identified in breast carcinoma and has since been documented in a variety of tumors including chondrosarcomas, basal cell carcinoma, and malignant melanoma. In addition it has been detected in inflammatory diseases such as atherosclerosis, in wound healing, and in destructive joint diseases such OA and RA. MMP13 degrades the native helix of several types of fibrillar collagen, and extracellular matrix proteins in vitro. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:500-2000 Immunohistochemistry: 1:50-200 Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice