216595
HRP-1 (HRP1, Hepatoma-derived Growth Factor, Related Protein 1)
Size
£704.00
- SKU:
- 216595
- Application:
- WB
- Purity:
- Purified by Protein G affinity chromatography from culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rat
- Immunogen:
- Recombinant protein corresponding to mouse Hepatoma-derived growth factor, Related Protein 1.
- Clone:
- 14L499
- Format:
- Supplied as a lyophilized powder in PBS. Reconstitute with 200ul sterile PBS.
- Extra Details:
- Hepatoma-derived growth factor (HDGF)-related protein (HRP)-1, a member of the HDGF gene family, showed testis-specific expression in mice. HRP-1 expression in spermatogenesis was analyzed in the testis of normal and azoospermic mice by Northern blot and immunohistochemistry. HRP-1 gene message was not expressed in the ovary and its product was detected only in the nuclei of germ cells, not in somatic cells. The HRP-1 gene is expressed through pachytene spermatocyte to round spermatid. HRP-1 gene expression was not detected in the testis of cryptorchid mice or in some strains of mutant mice. These findings suggest that the testis-specific HRP-1 gene may play an important role in the phase around meiotic cell division. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:500-1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice