HB-EGF (Heparin Binding EGF-like Growth Factor)

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£691.00

SKU:: 216583
Purity:: Purified by Protein G affinity chromatography from culture supernatant.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to human HB-EGF
Clone:: 14L487
Format:: Supplied as a lyophilized powder in PBS. Reconstitute with 200ul sterile PBS.
Extra Details:: Heparin-binding EGF-like Growth Factor (HB-EGF) is an EGF family growth factor that is synthesized as a transmembrane precursor (proHB-EGF). ProHB-EGF, which contains one EGF-like domain, is subject to variable O-linked glycosylation and N-terminal truncation. In human, this precursor is the cellular receptor for diphtheria toxin. ADAM12 or ADAM17-mediated cleavage of proHB-EGF releases a 22 kD soluble HB-EGF; the cytoplasmic fragment functions as a transcriptional repressor. HB-EGF directly activates EGF R and ErbB4 and promotes the development of many tissues. Human HB-EGF shares 77% aa sequence identity with mouse and rat HB-EGF. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:500-1000 Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice